Eukaryotic initiation factor 4B and the poly(A)-binding protein bind eIF4G competitively
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چکیده
منابع مشابه
Eukaryotic initiation factor 4B and the poly(A)-binding protein bind eIF4G competitively
The eukaryotic translation initiation factor (eIF) 4G functions as a scaffold protein that assembles components of the translation initiation complex required to recruit the 40S ribosomal subunit to an mRNA. Although many eukaryotes express two highly similar eIF4G isoforms, those in plants are highly divergent in size and sequence from one another and are referred to as eIF4G and eIFiso4G. Alt...
متن کاملHuman eukaryotic translation initiation factor 4G (eIF4G) possesses two separate and independent binding sites for eIF4A.
Mammalian translation initiation factor 4F (eIF4F) consists of three subunits, eIF4A, eIF4E, and eIF4G. eIF4G interacts directly with both eIF4A and eIF4E. The binding site for eIF4E is contained in the amino-terminal third of eIF4G, while the binding site for eIF4A was mapped to the carboxy-terminal third of the molecule. Here we show that human eIF4G possesses two separate eIF4A binding domai...
متن کاملStructural and functional similarities between the central eukaryotic initiation factor (eIF)4A-binding domain of mammalian eIF4G and the eIF4A-binding domain of yeast eIF4G.
The translation eukaryotic initiation factor (eIF)4G of the yeast Saccharomyces cerevisiae interacts with the RNA helicase eIF4A (a member of the DEAD-box protein family; where DEAD corresponds to Asp-Glu-Ala-Asp) through a C-terminal domain in eIF4G (amino acids 542-883). Mammalian eIF4G has two interaction domains for eIF4A, a central domain and a domain close to the C-terminus. This raises t...
متن کاملRecycling of messenger RNA cap-binding proteins mediated by eukaryotic initiation factor 4B.
The ability of polypeptide components of eukaryotic initiation factor (eIF) 4F to bind to the m7G cap of an mRNA, to be released from that mRNA, and then to rebind to the cap of a second mRNA has been investigated. The release and rebinding steps have been termed "recycling." It was found that eIF-4B stimulates the recycling of the 24-26 kDa (p24) component of eIF-4F, and perhaps of other compo...
متن کاملInitiation factor eIF-4B (IF-M3)-dependent recognition and translation of capped versus uncapped eukaryotic mRNAs.
Translation of capped and uncapped eukaryotic mRNAs is stimulated by addition of eIF-4B to an mRNA-dependent reticulocyte lysate system. m7G5 ppp inhibits translation of capped but not uncapped mRNAs and reduces translation of capped vaccinia mRNA to the level obtained with uncapped vaccinia mRNA. Exogenous eIF-4B but no other initiation factor reverses inhibition of protein synthesis by m7G5'p...
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ژورنال
عنوان ژورنال: Translation
سال: 2013
ISSN: 2169-0731
DOI: 10.4161/trla.24038